Services

Image
Preparative HPLC:
For the purification or isolation of the desirable products from the complex mixture of extracts or solutions.Amount of the purified compounds ranges from few micrograms to milligram. Examples includesenzymes isolation, extraction of specific phytoconstituent from the herbal extracts.

 

Analytical HPLC:
For the purification or isolation of the desirable products from the complex mixture of extracts or solutions.Amount of the purified compounds ranges from few micrograms to milligram. Examples includesenzymes isolation, extraction of specific phytoconstituent from the herbal extracts.

 

Q-trap Mass Spectrometry:
It is an excellent tool for the sensitive analysis of small molecules(<1000 Da). Q- trap mass spectrometry experiments:
  • Untargeted analysis: wide ranging analysis of entire measurable analytes from the samples.
  • Targeted analysis: measures only the desirable metabolites or analytes of interest.

 

Sample preparations:
  • Solid phase extractions
  • Liquid – Liquid extraction
  • Derivatization
  • Ultra-filtration
  • Selective precipitation

 

Reference:

Gupta H, Jain S, Mathur R, Mishra P, Mishra AK, Velpandian T. Sustained oculardrug delivery from a temperature and pH triggered novel in situ gel system. Drug Deliv. 2007 Nov;14(8):507-15. PubMed PMID: 18027180.

Velpandian T, Jaiswal J, Bhardwaj RK, Gupta SK. Development and validation of a new high-performance liquid chromatographic estimation method of meloxicam in biological samples. J Chromatogr B Biomed Sci Appl. 2000 Feb 11;738(2):431-6.PubMed PMID: 10718662.

Senthilkumari S, Velpandian T, Biswas NR, Saxena R, Ghose S. Evaluation of the modulation of P-glycoprotein (P-gp) on the intraocular disposition of its substrate in rabbits. Curr Eye Res. 2008 Apr;33(4):333-43. doi: 10.1080/02713680802015720. PubMed PMID: 18398708.

Mukherjee A, Velpandian T, Singla M, Kanhiya K, Kabra SK, Lodha R.Pharmacokinetics of isoniazid, rifampicin, pyrazinamide and ethambutol in Indian children. BMC Infect Dis. 2015 Mar 14;15:126. doi: 10.1186/s12879-015-0862-7.PubMed PMID: 25887748; PubMed Central PMCID: PMC4373095.

Velpandian T, Jasuja R, Bhardwaj RK, Jaiswal J, Gupta SK. Piperine in food:interference in the pharmacokinetics of phenytoin. Eur J Drug MetabPharmacokinet. 2001 Oct-Dec;26(4):241-7. PubMed PMID: 11808866.